Subjective experience along with physiological activity are fundamental components of emotional responding. We present a publicly available dataset of psychophysiological responses to positive and negative emotions of 1157 healthy participants, collected across seven studies. In our studies were continuously recorded affect and physiological activity during resting baseline and emotional responding. We recorded physiological responses using electrocardiography (EKG), impedance cardiography (ICG), electrodermal activity (EDA), photoplethysmography (PPG, the blood pressure measures), respiratory, and temperature sensors. In our studies, we elicited emotions with films, pictures, speech preparation, and expressive writing. We studied a wide range of positive and negative emotions, including: amusement, anger, disgust, excitement, fear, gratitude, sadness, tenderness, and threat. To the best of our knowledge, Psychophysiology of Positive and Negative Emotions (POPANE) database is the largest, consistent psychophysiological dataset on emotions ever collected and publicly shared. We hope that POPANE will provide individuals, companies, and laboratories with the data they need to perform their own analyses, corroborate their results, and create robust psychophysiological models of emotions.